Anti-Human CD274 Recombinant Antibody (TAB-417CQ)

CAT#: TAB-417CQ

Recombinant monoclonal antibody to CD274. This antibody is a human IgG1 monoclonal antibody that targets and binds to PD-L1, a protein that is often overexpressed on the surface of cancer cells and immune cells in the tumor microenvironment.

Tested Data Gene Expression
Figure 1 Anti-Human CD274 Antibody (TAB-417CQ) in ELISA Figure 2 Anti-Human CD274 Antibody (TAB-417CQ) in WB Figure 3 Anti-Human CD274 Antibody (TAB-417CQ) in DB
Figure 1 IF staining of human cell line U-251 MG Figure 2 IF staining of human cell line BJ Figure 3 IF staining of human cell line U-2 OS Figure 4 Colon Figure 5 Placenta Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG1, κ
  • Specificity
  • Human CD274
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IHC, FC, IP, IF, Inhib, FuncS
  • Conjugate
  • Unconjugated
  • Related Disease
  • Cancers, non-small cell lung (NSCLC), Solid tumors, bladder, urothelial

Product Property

  • Purity
  • > 95% as determined by SDS-PAGE
  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • The CD274 antibody has been reported in applications of ELISA, IHC, FC, IP, IF, Inhib, FuncS.

Target

  • Alternative Names
  • Programmed cell death 1 ligand 1; B7H1; B7-H1; PDL1; PD-L1; PDCD1L1; B7 homolog 1; B7 homologue 1
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Breast cancer biomarkers at key points during disease progression Emily Roberts
12.Sep,23
Ideal for Durvalumab Studies
The Durvalumab has been excellent for our PD-L1 studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
Breast cancer biomarkers at key points during disease progression Brian Anderson
21.Oct,22
Effective in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for PD-L1 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
Breast cancer biomarkers at key points during disease progression Jessica Moore
17.May,21
Consistent and High-Quality
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for PD-L1 have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Q&As

  1. Is the anti-Durvalumab therapeutic antibody suitable for use in Western blotting?

    A: Yes, the anti-Durvalumab therapeutic antibody (TAB-417CQ) is suitable for use in Western blotting. It provides specific binding to Durvalumab, allowing for reliable detection in Western blot assays.

  2. What are the storage recommendations for the anti-Durvalumab therapeutic antibody?

    A: The recommended storage condition for the anti-Durvalumab therapeutic antibody (TAB-417CQ) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

  3. Can the anti-Durvalumab therapeutic antibody be used in immunoprecipitation assays?

    A: Yes, the anti-Durvalumab therapeutic antibody (TAB-417CQ) can be used in immunoprecipitation assays. It provides specific binding to Durvalumab, enabling the successful precipitation of the target protein from complex mixtures.

  4. Is the anti-Durvalumab therapeutic antibody effective in ELISA applications?

    A: Yes, the anti-Durvalumab therapeutic antibody (TAB-417CQ) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of Durvalumab.

  5. What is the optimal dilution for using the anti-Durvalumab therapeutic antibody in immunofluorescence?

    A: The optimal dilution for using the anti-Durvalumab therapeutic antibody (TAB-417CQ) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

  1. Li, Yumei, et al. "A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells." Translational Oncology 21 (2022): 101424. https://doi.org/10.1016/j.tranon.2022.101424
    This study explores the creation and efficacy of a novel multifunctional antibody, anti-PD-L1-CD16a-IL15, designed to target and kill PD-L1-overexpressing tumor cells. The researchers utilized recombinant human PD-L1-immunized llamas to develop anti-PD-L1 single-domain antibodies, which were then linked with anti-CD16a single-domain antibodies and the IL-15/IL-15Ra complex. This fusion protein, expressed and purified from HEK-293F cells, demonstrated significant in vitro and in vivo antitumor activity. It recruits T cells and activates NK cells to specifically target and kill PD-L1-positive tumor cells, showing enhanced efficacy over other antibody constructs in both cell cultures and xenograft models.
    Creative Biolabs played a crucial role in this research by supplying several key reagents. Specifically, they provided the durvalumab antibody (Cat#: TAB-417CQ) used for comparison in cell proliferation assays and cytotoxicity studies. The comprehensive testing of anti-PD-L1-CD16a-IL15, including binding affinity, immune cell recruitment, and tumor inhibition, was facilitated by these high-quality reagents from Creative Biolabs. The products contributed to the study's ability to demonstrate the potential of anti-PD-L1-CD16a-IL15 as a potent cancer immunotherapy.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-417CQ, RRID: AB_3111930)

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Biosimilar Overview

Please refer to Durvalumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Durvalumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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VS-0425-YC608 Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608) Block, ELISA, FC, Cell-uptake
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